Journal: Nanomaterials

Article Title: In Vivo Evaluation of Bone Regenerative Capacity of the Novel Nanobiomaterial: β-Tricalcium Phosphate Polylactic Acid-co-Glycolide (β-TCP/PLLA/PGA) for Use in Maxillofacial Bone Defects

doi: 10.3390/nano14010091

Figure Lengend Snippet: β-TCP/PLLA/PGA (up) and β-TCP (down) scaffolds. β-TCP/PLLA/PGA electrospun cotton-like fibers ( upper left ) and SEM images of the fibers ( from left to right ): ×50, ×400, and ×2000 magnifications. β-TCP bone substitute block ( lower left ) and SEM images of β-TCP block ( from left to right ): ×50, ×100, and ×500 magnifications.

Article Snippet: Staples et al. studied the effect of electrospun mats on periodontal regeneration and noted that periostin deposition follows the fiber architecture, as in our study [ ].

Techniques: Blocking Assay

Journal: Nature Communications

Article Title: Intermittent scavenging of storage lesion from stored red blood cells by electrospun nanofibrous sheets enhances their quality and shelf-life

doi: 10.1038/s41467-022-35269-3

Figure Lengend Snippet: a Stored RBCs produce storage lesion or damage-associated molecular patterns (DAMPs) such as DNA, nucleosomes, heme (Hb), iron, and polyunsaturated fatty acids (PUFAs). Transfusion of DAMPs containing RBCs could lead to transfusion-related complications, including systemic inflammation and organ injury. The presence of DAMPs progressively reduces the quality of stored RBCs and limits their shelf-life. All DAMPs consist of positive or negative charge entities. Therefore, in our approach , we designed hemocompatible polymers poly-acridine and poly-taurine composed of complementary cationic and anionic charges, respectively. Using these polymers, charge-bearing electrospun nanofibrous sheets ( Tau -NFS and Acr -NFS) were prepared to scavenge charged DAMPs from stored RBCs, which remarkably enhanced the quality and shelf-life of stored RBCs. b Scanning electron microscopy images of Tau- NFS and Acr- NFS reveal the presence of nanofibrous mesh with a high aspect ratio (scale bar = 2 µm, representative images were selected from 15 images from three independent experiments). c Surface charge of Tau- NFS and Acr- NFS indicate that Tau- NFS has a net negative charge (−19.7 mV) and Acr- NFS has a net positive charge (+36.7 mV). d Contact angle measurements showed that surfaces of Tau- NFS and Acr- NFS are hydrophobic, which could reduce the absorption of aqueous components during the treatment of stored blood. e SEM images of Tau- NFS and Acr- NFS post-treatment with stored RBCs reveal that except for deposition of DAMPs, no cells have adhered to the NFS (scale bar = 10 µm, representative images were selected from 15 images from three independent experiments). f Hemolysis assay revealed that <1% hemolysis was observed upon treatment with Tau-Acr NFS. Data are mean ± s.d. ( n = 3, from independent experiments). Source data are provided as a Source Data file.

Article Snippet: Dried electrospun mats were mounted on aluminum stubs and sputter-coated with gold (Pelco® SC-7 Auto sputter coater).

Techniques: Electron Microscopy, Hemolysis Assay